![]() ![]() Immunoblot analysis of whole cell extracts treated with RFWD3 siRNAs in the absence or presence of HA-RFWD3 WT or CA mutant. Varying concentrations (25%, 50%, and 100%) of whole-cell extracts treated with control siRNA (siGl3) are shown to provide information on the percentage of knockdown and the reduction of protein levels. Immunoblot analysis of whole cell extracts from cells treated with RFWD3 siRNAs in the absence or presence of p53 siRNA. Varying concentrations (25%, 50%, and 100%) of whole cell extracts treated with control siRNA (siGl3) are shown to provide information on the percentage of knockdown and the reduction of protein levels. Immunoblot analysis of whole cell extracts from HCC38 cells treated with control or RFWD3 siRNAs. Immunoblot analysis of whole cell extracts from HCT116 WT (d) or p53 null (e) cells treated with RFWD3 siRNAs. ![]() Immunoblot analysis of whole cell extracts from HCT116 WT (a), p53 null (b), or p21 null (c) cells treated with RFWD3 siRNAs. RFWD3 loss results in p53-dependent reduction of ORC/ORCA. S1 represents cytosolic fraction, S2 represents nuclear soluble fraction, P represents chromatin insoluble fraction Immunoblot analysis of chromatin fractionation from cells treated with control, Orc1 or Orc2 siRNAs. Immunoblot analysis of whole cell extracts from cells treated with control, Orc1 or Orc2 siRNAs. Immunoblot analysis of HCT116 p53 null whole cell extracts transfected with HA-RFWD3 WT or CA mutant, in the absence or presence of T7-Orc2, Nutilin-3, or MG132. ![]() Immunoblot analysis of whole cell extracts (HCT116 WT or p21 null) transfected with HA-RFWD3 WT or CA mutant, in the absence or presence of T7-Orc2. ![]() “*”denotes cross-reacting band and loading control. Immunoblot analysis of HA-RFWD3 expression level in HCT116 WT, p21 null, and p53 null cells. P53-dependent stabilization of RFWD3 by ORC. ![]()
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